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A high dose KRP203 induces cytoplasmic vacuoles associated with altered phosphoinositide segregation and endosome expansion

Satoshi Kofuji, Kara Wolfe, Kazutaka Sumita, Shun Kageyama, Hirofumi Yoshino, Yoshihisa Hirota, Aki Ogawa-Iio, Hirotaka Kanoh, Mika Sasaki, Kaori Kofuji, Mindy I. Davis, Rajan Pragani, Min Shen, Matthew B. Boxer, Fubito Nakatsu, Kiyomi Nigorikawa, Takehiko Sasaki, Koh Takeuchi, Toshiya Senda, Seong M. Kim,Atsuo T. Sasaki

J​ournal:

Biochem. Biophys. Res. Commun.

2024

Year:

Vol.&Pages:

in press

​DOI :

10.1016/j.bbrc.2024.149981

​PMID:

​Pubmed URL:

Homogeneous luminescent quantitation of cellular guanosine and adenosine triphosphates (GTP and ATP) using QT-LucGTP&ATP assay

Kopra K, Mahran R, Yli-Hollo T, Tabata S, Vuorinen E, Fujii Y, Vuorinen I, Ogawa-Iio A, Hirayama A, Soga T, Sasaki AT, Härmä H.

J​ournal:

Anal Bioanal Chem.

2023

Year:

Vol.&Pages:

415(27)

6689-6700

​DOI :

10.1007/s00216-023-04944-9

37714971

​PMID:

​Pubmed URL:

Multimodal action of KRP203 on phosphoinositide kinases in vitro and in cells

Yoshiki Ikeda, Mindy I. Davis, Kazutaka Sumita, Yuxiang Zheng, Satoshi Kofuji, Mika Sasaki, Yoshihisa Hirota, Rajan Pragani, Min Shen, Matthew B. Boxer, Koh Takeuchi, Toshiya Senda, Anton Simeonov, Atsuo T. Sasaki

J​ournal:

Biochemical and Biophysical Research Communications

2023

Year:

Vol.&Pages:

679

116-121.

​DOI :

10.1016/j.bbrc.2023.08.050

37683456

​PMID:

​Pubmed URL:

Functional molecular evolution of a GTP sensing kinase: PI5P4Kβ

Takeuchi K, Senda M, Ikeda Y, Okuwaki K, Fukuzawa K, Nakagawa S, Sasaki M, Sasaki AT, Senda T.

J​ournal:

FEBS J

2023

Year:

Vol.&Pages:

290(18)

4419-4428

​DOI :

10.1111/febs.16763

36856076

​PMID:

​Pubmed URL:

IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia.

Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, Asada S, Fujino T, Fukushima T, Yonezawa T, Tanaka Y, Fukuyama T, Tsuchiya A, Shikata S, Iwamura H, Kinouchi C, Komatsu K, Yamasaki S, Shibata T, Sasaki AT, Schibler J, Wunderlich M, O'Brien E, Mizukawa B, Mulloy JC, Sugiura Y, Takizawa H, Shibata T, Miyake K, Kitamura T, Goyama S.

J​ournal:

EMBO Mol Med.

2023

Year:

Vol.&Pages:

15(1)

e15631

​DOI :

10.15252/emmm.202115631.

36453131

​PMID:

​Pubmed URL:

Beyond Warburg: LDHA activates RAC for tumour growth.

Osaka N, Sasaki AT*

J​ournal:

Nat Metab

2022

Year:

Vol.&Pages:

4(12)

1623-1625

​DOI :

10.1038/s42255-022-00709-3.

36536138.

​PMID:

​Pubmed URL:

The GTP responsiveness of PI5P4Kβ evolved from a compromised trade-off between activity and specificity.

Takeuchi K*, Ikeda Y, Senda M, Harada A, Okuwaki K, Fukuzawa K, Nakagawa S, Yu HY, Nagase L, Imai M, Sasaki M, Lo YH, Ito D, Osaka N, Fujii Y, Sasaki AT*, Senda T*. *Co-correspondence

J​ournal:

Structure

2022

Year:

Vol.&Pages:

S0969-2126(22)

00131-9

​DOI :

10.1016/j.str.2022.04.004.

35504278

​PMID:

​Pubmed URL:

Epigenetic upregulation of Schlafen11 renders WNT-and SHH-activated medulloblastomas sensitive to cisplatin.

Nakata S, Murai J, Okada M, Takahashi H, Findlay TH, Malebranche K, Parthasarathy A, Miyashita S, Gabdulkhaev R, Benkimoun I, Druillennec S, Chabi S, Hawkins E, Miyahara H, Tateishi K, Yamashita S, Yamada S, Saito T, On J, Watanabe J, Tsukamoto Y, Yoshimura J, Oishi M, Nakano T, Imamura M, Imai C, Yamamoto T, Takeshima H, Sasaki AT, Rodriguez FJ, Nobusawa S, Varlet P, Pouponnot C, Osuka S, Pommier Y, Kakita A, Fujii Y, Raabe EH, Eberhart CG, Natsumeda M.

J​ournal:

Neuro Oncol

2022

Year:

Vol.&Pages:

25(5)

899-912

​DOI :

10.1093/neuonc/noac243.

36273330

​PMID:

​Pubmed URL:

Schlafen 11 predicts response to platinum-based chemotherapy in gastric cancers.

Takashima T, Taniyama D, Sakamoto N, Yasumoto M, Asai R, Hattori T, Honma R, Thang PQ, Ukai S, Maruyama R, Harada K, Kuraoka K, Tanabe K, Sasaki AT, Ohdan H, Morii E, Murai J, Yasui W.

J​ournal:

Br J Cancer

2021

Year:

Vol.&Pages:

125(1)

65-77

​DOI :

10.1038/s41416-021-01364-3

33785877

​PMID:

​Pubmed URL:

TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.

Nakasuka F, Tabata S, Sakamoto T, Hirayama A, Ebi H, Yamada T, Umetsu K, Ohishi M, Ueno A, Goto H, Sugimoto M, Nishioka Y, Yamada Y, Tomita M, Sasaki AT, Yano S, Soga T.

J​ournal:

Commun Biol

2021

Year:

Vol.&Pages:

4(1)

782

​DOI :

10.1038/s42003-021-02323-7.

34168290

​PMID:

​Pubmed URL:

SI-MOIRAI:A new method to identify and quantify the metabolic fate of nucleotides. 

Ikeda Y, Hirayama A, Kofuji S, Hirota Y, Kamata R, Osaka N, Fujii Y, Sasaki M, Ikeda S, Smith EP, Bachoo R, Soga T, Sasaki AT*.

J​ournal:

J Biochem

2022

Year:

Vol.&Pages:

170(6)

699-711

​DOI :

10.1093/jb/mvab077.

34244779

​PMID:

​Pubmed URL:

Mycophenolate mofetil hepatotoxicity associated with mitochondrial abnormality in liver transplant recipients and mice.

Wallen M, Mitsinikos T, Yanni G, Sasaki AT, Thomas D. J.

J​ournal:

Ped. Gastro. Nutri.

2021

Year:

Vol.&Pages:

73(4)

463-470

​DOI :

10.1097/MPG.0000000000003171.

34016874

​PMID:

​Pubmed URL:

Reuse of Molecules for Glioblastoma Therapy.

Koehler A, Karve A, Desai P, Arbiser J, Plas DR, Qi X, Read RD, Sasaki AT, Gawali VS, Toukam DK, Bhattacharya D, Kallay L, Pomeranz Krummel DA, Sengupta S.

J​ournal:

Pharmaceuticals (Basel)

2021

Year:

Vol.&Pages:

14(2)

99

​DOI :

10.3390/ph14020099.

33525329

​PMID:

​Pubmed URL:

Targeting Energy Metabolism to Overcome Therapeutic Resistance of Glioblastoma and Tumor-associated Edema.(Review)

Biplab Dasgupta, Yoshihisa Hirota, Yuki Fujii, Natsuki Osaka, Doshun Ito, David R. Plas, Atsuo T. Sasaki*.

J​ournal:

In: Gliomas. Brisbane (AU): Exon Publications

2021

Year:

Vol.&Pages:

Chapter 7

​DOI :

10.36255/exonpublications.gliomas.2021.chapter7

34038050

​PMID:

​Pubmed URL:

Emerging Roles of Wild-type and Mutant IDH1 in Growth, Metabolism and Therapeutics of Glioma. (Review)

Garrett M, Fujii Y, Osaka N, Ito D, Hirota Y, Sasaki AT*

J​ournal:

In: Gliomas [Internet]. Brisbane (AU)

2021

Year:

Vol.&Pages:

Chapter 4

​DOI :

10.36255/exonpublications.gliomas.2021.chapter4

34038049

​PMID:

​Pubmed URL:

Integration of Molecular Analysis, Cutting-edge Mouse Genetic Models and Proton Therapy to Improve Outcomes for Glioma Patients.(Review)

Soma Sengupta, Luke E. Pater, Daniel Pomeranz Krummel, Bruce J. Aronow, Yoshihisa Hirota, Timothy N. Phoenix, Atsuo T. Sasaki*

J​ournal:

In: Gliomas. Brisbane (AU): Exon Publications

2021

Year:

Vol.&Pages:

Chapter 5

​DOI :

10.36255/exonpublications.gliomas.2021.chapter5

34038048

​PMID:

​Pubmed URL:

Divergent Mechanisms Activating RAS and Small GTPases Through Post-translational Modification. (Review)

Osaka N, Hirota Y, Ito D, Ikeda Y, Kamata R, Fujii Y, Chirasani VR, Campbell SL, Takeuchi K, Senda T, Sasaki AT*.

J​ournal:

Front Mol Biosci

2021

Year:

Vol.&Pages:

8

707439

​DOI :

10.3389/fmolb.2021.707439.

34307463

​PMID:

​Pubmed URL:

Metabolic compartmentalization at the leading edge of metastatic cancer cells. (Review)

Kamata R, Coutinho K, Inoue T, Sasaki AT*.

J​ournal:

Front Oncol

2020

Year:

Vol.&Pages:

10

554272

​DOI :

10.3389/fonc.2020.554272.

33224873

​PMID:

​Pubmed URL:

GTP Metabolic Reprogramming by IMPDH2: Unlocking Cancer Cells' Fueling Mechanism.

Kofuji S, Sasaki AT*.

J​ournal:

J Biochem

2020

Year:

Vol.&Pages:

168(4)

319-328

​DOI :

10.1093/jb/mvaa085.

32702086

​PMID:

​Pubmed URL:

Activity-specificity trade-off gives PI5P4Kβ a nucleotide preference to function as a GTP-sensing kinase.

Takeuchi K, Ikeda Y, Senda M, Harada A, Okuwaki K, Fukuzawa K, Nakagawa S, Yu H, Nagase L, Imai M, Sasaki M, Lo YH, Sasaki AT*, Senda T*.

J​ournal:

bioRxiv

2020

Year:

Vol.&Pages:

​DOI :

​PMID:

​Pubmed URL:

Structure-Activity Relationship Study of Covalent Pan-phosphatidylinositol 5-Phosphate 4-Kinase Inhibitors.

Manz TD, Sivakumaren SC, Yasgar A, Hall MD, Davis MI, Seo HS, Card JD, Ficarro SB, Shim H, Marto JA, Dhe-Paganon S, Sasaki AT, Boxer MB, Simeonov A, Cantley LC, Shen M, Zhang T, Ferguson FM, Gray NS.

J​ournal:

ACS Med Chem Lett.

2019

Year:

Vol.&Pages:

11(3)

346-352

​DOI :

10.1021/acsmedchemlett.9b00402.

32184968

​PMID:

​Pubmed URL:

Anti-Tumor Potential of IMP Dehydrogenase Inhibitors: A Century-Long Story.(Review)

Naffouje R, Grover P, Yu H, Sendilnathan A, Wolfe K, Majd N, Smith EP, Takeuchi K, Senda T, Kofuji S, Sasaki AT*.

J​ournal:

Cancers (Basel)

2019

Year:

Vol.&Pages:

11(9)

pii: E1346

​DOI :

10.3390/cancers11091346.

31514446

​PMID:

​Pubmed URL:

IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma.

Kofuji S, Yoshino H, Sumita K, Malhotra A, Sasaki M, Kaori K, Takiko D, Majd N, Hirayama A, Soga T, Pater L, Gozal Y, Wse-Draper T, Gogela S, Kendler A, Warnick RE, Chow L, Horbinski C, Warren Kofuji, S., Hirayama, A., Eberhardt, A., Kawaguchi, R., Sugiura, Y., Sampetrean, O., Ikeda, Y., Warren, M., Sakamoto, N., Kitahara, S., Yoshino, H., Yamashita, D., Sumita, K., Wolfe, K., Lange, L., Ikeda, S., Shimada, H., Minami, N., Malhotra, A., Morioka, S., Ban, Y., Asano, M., Ramkissoon, A., Chow, L. M. L., Kiyokawa, J., Mashimo, T., Lucey, G., Mareninov, S., Ozawa, T., Onishi, N., Okumura, K., Terakawa, J., Daikoku, T., Wise-Draper, T., Majd, N., Kofuji, K., Sasaki, M., Mori, M., Kanemura, Y., Smith, E., Anastasiou, D., Wakimoto, H., Holland, E. C., Yong, W. H., Horbinski, C., Nakano, I., Deberardinis, R. J., Bachoo, R. M., Mischel, P. S., Yasui, W., Suematsu, M., Saya, H., Soga, T., Grummt, I., Bierhoff, H., and Sasaki, AT*.

J​ournal:

Nat Cell Biol.

2019

Year:

Vol.&Pages:

21(8)

1003-1014.

​DOI :

10.1038/s41556-019-0363-9.

31371825

​PMID:

​Pubmed URL:

Identification of lysine methylation in the core GTPase domain by GoMADScan.

Yoshino H, Yin G, Kawaguchi R, Popov KI, Temple B, Sasaki M, Kofuji S, Wolfe K, Kofuji K, Okumura K, Randhawa J, Malhotra A, Majd N, Ikeda Y, Shimada H, Kahoud ER, Haviv S, Iwase S, Asara JM, Campbell SL, Sasaki AT*.

J​ournal:

PLoS One

2019

Year:

Vol.&Pages:

14(8)

e0219436

​DOI :

10.1371/journal.pone.0219436.

31390367

​PMID:

​Pubmed URL:

Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria.

Miller TE, Henkels KM, Huddleston M, Salisbury R, Hussain SM, Sasaki AT, Cho KJ.

J​ournal:

J Cell Sci

2019

Year:

Vol.&Pages:

132(16)

jcs231886

​DOI :

10.1242/jcs.231886

31331963

​PMID:

​Pubmed URL:

Dynamic Compartmentalization of Purine Nucleotide Metabolic Enzymes at Leading Edge in Highly Motile Renal Cell Carcinoma.

Wolfe, K., Kofuji, S., Yoshino, H., Sasaki, M., Okumura, K., and Sasaki, AT*.

J​ournal:

Biochem Biophys Res Commun

2019

Year:

Vol.&Pages:

516(1)

50-56

​DOI :

10.1016/j.bbrc.2019.05.190.

31196624

​PMID:

​Pubmed URL:

R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling.

Su R, Dong L, Li C, Nachtergaele S, Wunderlich M, Qing Y, Deng X, Wang Y, Weng X, Hu C, Yu M, Skibbe J, Dai Q, Zou D, Wu T, Yu K, Weng H, Huang H, Ferchen K, Qin X, Zhang B, Qi J, Sasaki AT, Plas DR, Bradner JE, Wei M, Marcucci G, Jiang X, Mulloy JC, Jin J, He C, Chen J.

J​ournal:

Cell

2018

Year:

Vol.&Pages:

172(1-2)

90-105

​DOI :

10.1016/j.cell.2017.11.031.

29249359

​PMID:

​Pubmed URL:

In-vitro evaluation of MPA-loaded electrospun coaxial fiber membranes for local treatment of glioblastoma tumor cells.

Han D, Sasaki M, Yoshino H, Kofuji S, AT Sasak AT, Steckl AJ.

J​ournal:

J. Drug Deli. Sci. Tech.

2017

Year:

Vol.&Pages:

7

45-50

​DOI :

​PMID:

​Pubmed URL:

Pharmacologic Targeting of S6K1 in PTEN-Deficient Neoplasia.

Liu H, Feng X, Ennis KN, Behrmann CA, Sarma P, Jiang TT, Kofuji S, Niu L, Stratton Y, Thomas HE, Yoon SO, Sasaki AT, Plas DR.

J​ournal:

Cell Rep

2017

Year:

Vol.&Pages:

18(9)

2088-2095

​DOI :

10.1016/j.celrep.2017.02.022

28249155

​PMID:

​Pubmed URL:

Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways.

Kim SM, Roy SG, Chen B, Nguyen TM, McMonigle RJ, McCracken AN, Zhang Y, Kofuji S, Hou J, Selwan E, Finicle BT, Nguyen TT, Ravi A, Ramirez MU, Wiher T, Guenther GG, Kono M, Sasaki AT, Weisman LS, Potma EO, Tromberg BJ, Edwards RA, Hanessian S, Edinger AL. Edinger.

J​ournal:

J. Clin. Invest.

2016

Year:

Vol.&Pages:

126

4088-4102

​DOI :

10.1172/JCI87148.

27669461

​PMID:

​Pubmed URL:

Use of multiple cryoprotectants to improve diffraction quality from protein crystals.

Senda M, Hayashi T, Hatakeyama M, Takeuchi K, Sasaki AT, and Senda T.

J​ournal:

Cryst. Growth Des.

2016

Year:

Vol.&Pages:

63(Pt 11)

1139-53

​DOI :

10.1107/S0907444907045040.

18007029

​PMID:

​Pubmed URL:

Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format.

Davis MI, Sasaki AT, Simeonov A.

J​ournal:

Methods Mol Biol

2016

Year:

Vol.&Pages:

1376

1-9

​DOI :

10.1007/978-1-4939-3170-5_1.

26552670

​PMID:

​Pubmed URL:

Structural reverse genetics study of the PI5P4Kβ-nucleotide complexes reveals the presence of the GTP bioenergetic system in mammalian cells.

Takeuchi K, Senda M, Lo YH, Kofuji S, Ikeda Y, Sasaki AT*, Senda T.

J​ournal:

FEBS J

2016

Year:

Vol.&Pages:

283(19)

3556-3562

​DOI :

10.1111/febs.13739.

27090388

​PMID:

​Pubmed URL:

The lipid kinase PI5P4Kβ is an intracellular GTP sensor for metabolism and tumorigenesis.

Sumita K, Lo YH, Takeuchi K, Senda M, Kofuji S, Ikeda Y, Terakawa J, Sasaki M, Yoshino H, Majd N, Zheng Y, Kahoud ER, Yokota T, Emerling BM, Asara JM, Ishida T, Locasale JW, Daikoku T, Anastasiou D, Senda T, Sasaki AT*.

J​ournal:

Mol Cell

2016

Year:

Vol.&Pages:

61(2)

187-98

​DOI :

10.1016/j.molcel.2015.12.011.

26774281

​PMID:

​Pubmed URL:

Degaradation of Activated K-Ras Orthologue via K-Ras Specific Lysine Residues is Required for Cytokinesis.

Sumita K, Yoshino H, Sasaki M, Majd N, Kahoud ER, Takahashi H, Takeuchi K, Kuroda T, Lee S, Charest PG, Takeda K, Asara JM, Firtel RA, Anastasiou D, Sasaki AT*.

J​ournal:

J Biol Chem

2014

Year:

Vol.&Pages:

289(7)

3950-9

​DOI :

10.1074/jbc.M113.531178.

24338482

​PMID:

​Pubmed URL:

A Review of the Potential Utility of Mycophenolate Mofetil as a Cancer Therapeutic.

Majd N, Sumita K, Yoshino H, Chen D, Terakawa J, Daikoku T, Kofuji S, Curry R, Wise-Draper T, Warnick RE, Guarnaschelli J and Sasaki AT*.

J​ournal:

J. Cancer Res.

2014

Year:

Vol.&Pages:

2014

Article ID 423401

​DOI :

​PMID:

​Pubmed URL:

Depletion of a Putatively Druggable Class of Phosphatidylinositol Kinases Inhibits Growth of p53-Null Tumors.

Emerling BM, Hurov JB, Poulogiannis G, Tsukazawa KS, Choo-Wing R, Wulf GM, Bell EL, Shim HS, Lamia KA, Rameh LE, Bellinger G, Sasaki AT, Asara JM, Yuan X, Bullock A, Denicola GM, Song J, Brown V, Signoretti S, Cantley LC.

J​ournal:

Cell

2013

Year:

Vol.&Pages:

155

844-857

​DOI :

10.1016/j.cell.2013.09.057.

24209622

​PMID:

​Pubmed URL:

Differences in the Regulation of K-Ras and H-Ras Isoforms by Monoubiquitination.

Baker R, Wilkerson EM, Sumita K, Isom DG, Sasaki AT, Dohlman HG, Campbell SL.

J​ournal:

J Biol Chem

2013

Year:

Vol.&Pages:

288(52)

36856-62

​DOI :

10.1074/jbc.C113.525691.

24247240

​PMID:

​Pubmed URL:

A homogeneous, high-throughput assay for phosphatidylinositol 5-phosphate 4-kinase with a novel, rapid substrate preparation.

Davis MI*, Sasaki AT*, Shen M, Emerling BM, Thorne N, Michael S, Pragani R, Boxer M, Sumita K, Takeuchi K, Auld DS, Li Z, Cantley LC, Simeonov A. (*These authors contributed equally to this work)

J​ournal:

PLoS One

2013

Year:

Vol.&Pages:

8(1)

e54127

​DOI :

10.1371/journal.pone.0054127.

23326584

​PMID:

​Pubmed URL:

The brain microenvironment negatively regulates miRNA-768-3p to promote K-ras expression and lung cancer metastasis.

Subramani A, Alsidawi S, Jagannathan S, Sumita K, Sasaki AT, Aronow B, Warnick RE, Lawler S, Driscoll JJ.

J​ournal:

Sci Rep

2013

Year:

Vol.&Pages:

3

2392

​DOI :

10.1038/srep02392.

23928793

​PMID:

​Pubmed URL:

Site-specific monoubiquitination activates Ras by impeding GTPase-activating protein function. (Featured in the Nat Rev Mol Cell Biol 2012 Dec 19. doi: 10.1038/nrm3500: Cell signalling: How monoubiquitylation keeps RAS active.)

Baker R, Lewis SM, Sasaki AT, Cantley LC, Kuhlman B, Dohlman HG, Campbell SL.

J​ournal:

Nat. Stru. Mol. Biol

2013

Year:

Vol.&Pages:

20

46-52

​DOI :

10.1038/nsmb.2430

23178454

​PMID:

​Pubmed URL:

Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.

Fujimura K, Sasaki AT, Anderson P.

J​ournal:

Nucleic Acids Res.

2012

Year:

Vol.&Pages:

40

8099-8110

​DOI :

10.1093/nar/gks566

22718973

​PMID:

​Pubmed URL:

Systemic Elevation of PTEN Induces a Tumor-Suppressive Metabolic State.

Garcia-Cao I, Song MS, Hobbs RM, Laurent G, Giorgi C, de Boer VC, Anastasiou D, Ito K, Sasaki AT, Rameh L, Carracedo A, Vander Heiden MG, Cantley LC, Pinton P, Haigis MC, Pandolfi PP.

J​ournal:

Cell

2012

Year:

Vol.&Pages:

149

49-62

​DOI :

10.1016/j.cell.2012.02.030

22401813

​PMID:

​Pubmed URL:

Inhibition of lung cancer growth: ATP citrate lyase knockdown and statin treatment leads to dual blockade of mitogen-actiated protein kinase (MAPK) and phosphatidylinositol-3- kinase (PI3K)/AKT pathways.

Hanai J, Doro N, Sasaki AT, Kobayashi S, Cantley LC, Seth P, Sukhatme VP.

J​ournal:

J. Cell Physiol.

2012

Year:

Vol.&Pages:

227(4)

1709-20

​DOI :

10.1002/jcp.22895

21688263

​PMID:

​Pubmed URL:

Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. (*Featured article in this issue: Science 2011;334:1219-20.)

Anastasiou D, Poulogiannis G, Asara JM, Bellinger G, Vander Heiden MG, Sasaki AT, Locasale JW and Cantley LC.

J​ournal:

Science

2011

Year:

Vol.&Pages:

334

1278-83

​DOI :

10.1126/science.1211485

22052977

​PMID:

​Pubmed URL:

Ubiquitination of Ras enhances activation and facilitates binding to PI 3-Kinase and Raf. (* Highlighted as the cover page and come with perspective Science Signaling. 2011;4:12.) (*This paper was evaluated and selected by the Faculty of 1000 Biology member)

Sasaki AT, Carracedo A, Locasale JW, Anastasiou D, Takeuchi K, Kahoud ER, Haviv S, Asara JM, Pandolfi PP, Cantley LC.

J​ournal:

Science Signaling.

2011

Year:

Vol.&Pages:

4

1-9

​DOI :

10.1126/scisignal.2001518

21386094

​PMID:

​Pubmed URL:

Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis.

Locasale JW, Grassian AR, Bass AJ, Heffron G, Metallo CM, Sharfi H, Sasaki AT, Anastasiou D, Vokes NI, Sasaki M, Ligon AH, Beroukhim R, Stephanopoulos G, Asara JM, Meyerson M, Wagner G, Brugge JS, Cantley LC and Vander Heiden MG.

J​ournal:

Nature Genetics

2011

Year:

Vol.&Pages:

43

869-74

​DOI :

10.1038/ng.890.

21804546

​PMID:

​Pubmed URL:

A fluorescent reporter of AMPK activity and cellular energy stress. (* Featured article in this issue of Cell Metabolism) (*This paper was evaluated and selected by the Faculty of 1000 Biology member)

Thou P, Zheng B, Hsu CH, Sasaki AT, Cantley LC.

J​ournal:

Cell Metabolism.

2011

Year:

Vol.&Pages:

13(4)

476-486

​DOI :

10.1016/j.cmet.2011.03.006.

21459332

​PMID:

​Pubmed URL:

Characterization of KRAS rearrangements in metastatic prostate cancer. (*Come with spotlight Cancer Discovery. 2011;1:12-13.)

Wang XS, Shankar S, Dhanasekaran SM, Ateeq B, Sasaki AT, Robinson D, Cao Q, Prensner JR, Yocum AK, Pflueger D, Wang R, Jing X, Fries DF, Asangani IA, Li Y, Cao X, Kumar-Sinha C, Kahoud ER, Omenn GS, Rubin MA, Gopalan A, Reuter V, Palanisamy N, Varambally S, Cantley LC, and Chinnaiyan AM.

J​ournal:

Cancer Discovery.

2011

Year:

Vol.&Pages:

1

35-43

​DOI :

10.1158/2159-8274.CD-10-0022

22140652

​PMID:

​Pubmed URL:

A Ras signaling complex controls the RasC-TORC2 pathway and directed cell migration.

Charest PG, Shen Z, Lakoduc A, Sasaki AT, Briggs S, Firtel RA.

J​ournal:

Dev. Cell

2010

Year:

Vol.&Pages:

18

737-479

​DOI :

10.1016/j.devcel.2010.03.017

20493808

​PMID:

​Pubmed URL:

Inhibition of mTORC1 leads to MAPK pathway activation through a PI3K-dependent feedback loop in human cancer.

Carracedo A, Ma L, Teruya-Feldstein J, Rojo F, Salmena L, Alimonti A, Egia A, Sasaki AT, Thomas G, Kozma SC, Papa A, Nardella C, Cantley LC, Baselga J, Pandolfi PP.

J​ournal:

J Clin Invest.

2008

Year:

Vol.&Pages:

118

3065-74

​DOI :

10.1172/JCI34739

18725988

​PMID:

​Pubmed URL:

G protein-independent Ras/PI3K/F-actin circuit regulates basic cell motility. (* Highlighted in J. Cell Biol. 2007;178:180.)

Sasaki AT, Janetopoulos C, Lee S, Charest PG, Takeda K Sundheimer LW, Meili R, Devreotes PN, Firtel RA.

J​ournal:

J. Cell Biol.

2007

Year:

Vol.&Pages:

178

185-191

​DOI :

10.1083/jcb.200611138

17635933

​PMID:

​Pubmed URL:

Regulation of chemotaxis by the orchestrated activation of Ras, PI3K, and TOR.

Sasaki AT, Firtel RA.

J​ournal:

Eur. J. Cell Biol.

2006

Year:

Vol.&Pages:

85(9-10)

873-95

​DOI :

10.1016/j.ejcb.2006.04.007.

16740339

​PMID:

​Pubmed URL:

Role of PI3 kinases in chemotaxis in dictyostelium.

Takeda K, Sasaki AT, Ha H, Seung HA, Firtel RA.

J​ournal:

J Biol Chem.

2007

Year:

Vol.&Pages:

283

11874-84

​DOI :

10.1074/jbc.M610984200

17331950

​PMID:

​Pubmed URL:

Suppressor of cytokine signaling 1 in lymphocytes regulates the development of intestinal inflammation in mice.

Inagaki-Ohara K, Sasaki A, Matsuzaki G, Ikeda T, Hotokezaka M, Chijiiwa K, Kubo M, Yoshida H, Nawa Y, Yoshimura A.

J​ournal:

Gut.

2006

Year:

Vol.&Pages:

55(2)

212-9

​DOI :

10.1136/gut.2004.062653

16120757

​PMID:

​Pubmed URL:

TOR complex 2 integrates cell movement during chemotaxis and signal relay in Dictyostelium.

Lee S, Comer FI, Sasaki A, McLeod IX, Duong Y, Okumura K, Yates JR, Parent CA, Firtel RA.

J​ournal:

Mol Biol Cell.

2005

Year:

Vol.&Pages:

16(10)

4572-83

​DOI :

10.1091/mbc.e05-04-0342

16079174

​PMID:

​Pubmed URL:

Finding the way: directional sensing and cell polarization through Ras signalling.

Sasaki AT, Firtel RA.

J​ournal:

Novartis Found Symp.

2005

Year:

Vol.&Pages:

269

73-87

​DOI :

16355536

​PMID:

​Pubmed URL:

Loss of mammalian Sprouty2 leads to enteric neuronal hyperplasia and esophageal achalasia.

Taketomi T, Yoshiga D, Taniguchi K, Kobayashi T, Nonami A, Kato R, Sasaki M, Sasaki A, Ishibashi H, Moriyama M, Nakamura K, Nishimura J, Yoshimura A.

J​ournal:

Nat Neurosci.

2005

Year:

Vol.&Pages:

8(7)

855-7

​DOI :

10.1038/nn1485

15937482

​PMID:

​Pubmed URL:

Activation and translocation of PKCdelta is necessary for VEGF-induced ERK activation through KDR in HEK293T cells.

Kuriyama M, Taniguchi T, Shirai Y, Sasaki A, Yoshimura A, Saito N.

J​ournal:

Biochem Biophys Res Commun.

2004

Year:

Vol.&Pages:

325(3)

843-51

​DOI :

10.1016/j.bbrc.2004.10.102

15541367

​PMID:

​Pubmed URL:

Localized Ras signaling at the leading edge regulates PI3K, cell polarity, and directional cell movement. (*This paper was evaluated and selected by the Faculty of 1000 Biology member)

Sasaki AT, Chun C, Takeda K, Firtel RA.

J​ournal:

J Cell Biol

2004

Year:

Vol.&Pages:

167(3)

505-18

​DOI :

10.1083/jcb.200406177

15534002

​PMID:

​Pubmed URL:

Spred-1 negatively regulates interleukin-3-mediated ERK/mitogen-activated protein (MAP) kinase activation in hematopoietic cells.

Nonami A, Kato R, Taniguchi K, Yoshiga D, Taketomi T, Fukuyama S, Harada M, Sasaki A, Yoshimura A.

J​ournal:

J Biol Chem

2004

Year:

Vol.&Pages:

279(50)

52543-51

​DOI :

10.1074/jbc.M405189200

15465815

​PMID:

​Pubmed URL:

Mucosal T cells bearing TCRgammadelta play a protective role in intestinal inflammation.

Inagaki-Ohara K, Chinen T, Matsuzaki G, Sasaki A, Sakamoto Y, Hiromatsu K, Nakamura-Uchiyama F, Nawa Y, Yoshimura A.

J​ournal:

J Immunol.

2004

Year:

Vol.&Pages:

173(2)

1390-8

​DOI :

10.4049/jimmunol.173.2.1390

15240735

​PMID:

​Pubmed URL:

Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1.

Sasaki A, Taketomi T, Kato R, Saeki K, Nonami A, Sasaki M, Kuriyama M, Saito N, Shibuya M, Yoshimura A.

J​ournal:

Cell Cycle.

2003

Year:

Vol.&Pages:

2(4)

281-282

​DOI :

10.1038/ncb978.

12717443

​PMID:

​Pubmed URL:

CIS1 interacts with the Y532 of the prolactin receptor and suppresses prolactin-dependent STAT5 activation.

Endo T, Sasaki A, Minoguchi M, Joo A, Yoshimura A.

J​ournal:

J Biochem (Tokyo)

2003

Year:

Vol.&Pages:

133(1)

109-13

​DOI :

10.1093/jb/mvg004

12761205

​PMID:

​Pubmed URL:

Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1. (*Highlighted in Nat. Cell Biol. 2003 5: 377-379)

Sasaki A, Taketomi T, Kato R, Saeki K, Nonami A, Sasaki M, Kuriyama M, Saito N, Shibuya M, Yoshimura A.

J​ournal:

Nat Cell Biol.

2003

Year:

Vol.&Pages:

5(5)

427-32

​DOI :

10.1038/ncb978

12717443

​PMID:

​Pubmed URL:

The N-terminal truncated isoform of SOCS3 translated from an alternative initiation AUG codon under stress conditions is stable due to the lack of a major ubiquitination site, Lys-6.

Sasaki A, Inagaki-Ohara K, Yoshida T, Yamanaka A, Sasaki M, Yasukawa H, Koromilas AE, Yoshimura A.

J​ournal:

J Biol Chem.

2003

Year:

Vol.&Pages:

278(4)

2432-6

​DOI :

10.1074/jbc.C200608200

12459551

​PMID:

​Pubmed URL:

SHP2 and SOCS3 contribute to Tyr-759-dependent attenuation of interleukin-6 signaling through gp130.

Lehmann U, Schmitz J, Weissenbach M, Sobota RM, Hortner M, Friederichs K, Behrmann I, Tsiaris W, Sasaki A, Schneider-Mergener J, Yoshimura A, Neel BG, Heinrich PC, Schaper F.

J​ournal:

J Biol Chem.

2003

Year:

Vol.&Pages:

278(1)

661-71

​DOI :

10.1074/jbc.M210552200

12403768

​PMID:

​Pubmed URL:

Identification of a dominant negative mutant of Sprouty that potentiates fibroblast growth factor- but not epidermal growth factor-induced ERK activation.

Sasaki A, Taketomi T, Wakioka T, Kato R, Yoshimura A.

J​ournal:

J Biol Chem

2001

Year:

Vol.&Pages:

276(39)

36804-8

​DOI :

10.1074/jbc.C100386200

11495895

​PMID:

​Pubmed URL:

Spred is a Sprouty-related suppressor of Ras signalling.

Wakioka T, *Sasaki A, Kato R, Shouda T, Matsumoto A, Miyoshi K, Tsuneoka M, Komiya S, Baron R, Yoshimura A. (*These authors contributed equally to this work)

J​ournal:

Nature.

2001

Year:

Vol.&Pages:

412(6847)

647-51

​DOI :

10.1038/35088082

11493923

​PMID:

​Pubmed URL:

JAB/SOCS1/SSI-1 is an interleukin-2-induced inhibitor of IL-2 signaling.

Sporri B, Kovanen PE, Sasaki A, Yoshimura A, Leonard WJ.

J​ournal:

Blood.

2001

Year:

Vol.&Pages:

97(1)

221-6

​DOI :

10.1182/blood.v97.1.221

11133764

​PMID:

​Pubmed URL:

CIS3/SOCS-3 suppresses erythropoietin (EPO) signaling by binding the EPO receptor and JAK2.

Sasaki A, Yasukawa H, Shouda T, Kitamura T, Dikic I, Yoshimura A.

J​ournal:

J Biol Chem.

2000

Year:

Vol.&Pages:

275(38)

29338-47.

​DOI :

10.1074/jbc.M003456200

10882725

​PMID:

​Pubmed URL:

CIS associates with the interleukin-2 receptor beta chain and inhibits interleukin-2-dependent signaling.

Aman MJ, Migone TS, Sasaki A, Ascherman DP, Zhu M, Soldaini E, Imada K, Miyajima A, Yoshimura A, Leonard WJ.

J​ournal:

J Biol Chem.

1999

Year:

Vol.&Pages:

274(42)

30266-72.

​DOI :

10.1074/jbc.274.42.30266

10514520

​PMID:

​Pubmed URL:

SOCS3 is essential in the regulation of fetal liver erythropoiesis.

Marine JC, McKay C, Wang D, Topham DJ, Parganas E, Nakajima H, Pendeville H, Yasukawa H, Sasaki A, Yoshimura A, Ihle JN.

J​ournal:

Cell.

1999

Year:

Vol.&Pages:

98(5)

617-27.

​DOI :

10.1016/s0092-8674(00)80049-5

10490101

​PMID:

​Pubmed URL:

7. Sasaki A, Yasukawa H, Suzuki A, Kamizono S, Syoda T, Kinjyo I, Sasaki M, Johnston JA, Yoshimura A. Cytokine-inducible SH2 protein-3 (CIS3/SOCS3) inhibits Janus tyrosine kinase by binding through the N-terminal kinase inhibitory region as well as SH2 domain. Genes Cells. 1999;4(6):339-51.

J​ournal:

Year:

Vol.&Pages:

​DOI :

​PMID:

​Pubmed URL:

6. Wakioka T, Sasaki A, Mitsui K, Yokouchi M, Inoue A, Komiya S, Yoshimura A. APS, an adaptor protein containing Pleckstrin homology (PH) and Src homology-2 (SH2) domains inhibits the JAK-STAT pathway in collaboration with c-Cbl. Leukemia. 1999;13(5):760-7.

J​ournal:

Year:

Vol.&Pages:

​DOI :

​PMID:

​Pubmed URL:

5. Yasukawa H, Misawa H, Sakamoto H, Masuhara M, Sasaki A, Wakioka T, Ohtsuka S, Imaizumi T, Matsuda T, Ihle JN, Yoshimura A. The JAK-binding protein JAB inhibits Janus tyrosine kinase activity through binding in the activation loop. EMBO J. 1999;18(5):1309-20.

J​ournal:

Year:

Vol.&Pages:

​DOI :

​PMID:

​Pubmed URL:

APS, an adaptor protein containing PH and SH2 domains, is associated with the PDGF receptor and c-Cbl and inhibits PDGF-induced mitogenesis.

Yokouchi M, Wakioka T, Sakamoto H, Yasukawa H, Ohtsuka S, Sasaki A, Ohtsubo M, Valius M, Inoue A, Komiya S, Yoshimura A.

J​ournal:

Oncogene.

1999

Year:

Vol.&Pages:

18(3)

759-67.

​DOI :

10.1038/sj.onc.1202326

9989826

​PMID:

​Pubmed URL:

CIS3 and JAB have different regulatory roles in interleukin-6 mediated differentiation and STAT3 activation in M1 leukemia cells.

Suzuki R, Sakamoto H, Yasukawa H, Masuhara M, Wakioka T, Sasaki A, Yuge K, Komiya S, Inoue A, Yoshimura A.

J​ournal:

Oncogene.

1998

Year:

Vol.&Pages:

17(17)

2271-8.

​DOI :

10.1038/sj.onc.1202143.

9811457

​PMID:

​Pubmed URL:

J​ournal:

Year:

Vol.&Pages:

​DOI :

​PMID:

​Pubmed URL:

*Publicized in Ehime News, Yamagata News; *Broadcasted by Cincinnati Public Radio

J​ournal:

Year:

Vol.&Pages:

​DOI :

​PMID:

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J​ournal:

Year:

Vol.&Pages:

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*Featured on the web of AAAS/ Eureka Alert, LONG ROOM, eCancer News, Specialty Pharmacy Times, Apple News, Research MOZ, Drug Target Review, Science Magazine, News Beezer, Agenparl, ETHIOPIA, Medical Xpress, Science Daily, Life Extension Advocacy Foundation, Asian Telegraph, Oncology Tribune, Qlifepro, Research-Japan

J​ournal:

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